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• Mar 13, 2019 · Subsequently, cells were cross-linked by adding formaldehyde (1% volume per volume final) for 8 min at room temperature and then quenched by adding glycine (200 mM final). After washing with PBS containing 20 mM sodium butyrate, 1 mM PMSF and 1 × protease inhibitor cocktail, cross-linked cells were stored in − 80 °C as dry pellet.
• We investigated the taxonomic status of the Indian forms of the Dendrelaphis pictus (Gmelin, 1789) group on the basis of multivariate analyses of morphological data taken from 176 museum specimens and two living specimens. A geographically isolated form from the Western Ghats, southwest India, is described as a new species. The subspecies Dendrelaphis pictus andamanensis (Anderson, 1871), an ...